MacFormat 1995 March

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MacBinary | 1994-10-14 | 84.9 KB | [STAK/TRJT]
open in:
MacOS 8.1 extracted |
Win98 extracted |
DOS extracted
view JSON data |
view as text

This file was processed as: MacBinary (archive/macBinary).

You can browse this item here: Protocol Book
Confidence	Program	Detection	Match Type	Support
`100%`	dexvert	HyperCard Stack `(archive/hypercard)`	idMeta	Supported
`10%`	dexvert	MacBinary `(archive/macBinary)`	fallback	Supported
`100%`	file	MacBinary II, busy, Sat Apr 10 14:59:30 1993, modified Fri Oct 14 10:23:40 1994, creator 'TRJT', type HyperCard stack, 63040 bytes "Protocol Book" , at 0xf6c0 23666 bytes resource	default (weak)
`99%`	file	data	default
`74%`	TrID	Mac HyperCard stack (MacBinary)	default
`25%`	TrID	MacBinary 2	default (weak)
`100%`	lsar	MacBinary	default
id metadata
key	value
macFileType	`[STAK]`
macFileCreator	`[TRJT]`
hex view
+--------+-------------------------+-------------------------+--------+--------+
|00000000| 00 0d 50 72 6f 74 6f 63 | 6f 6c 20 42 6f 6f 6b 00 |..Protoc|ol Book.|
|00000010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000040| 00 53 54 41 4b 54 52 4a | 54 04 00 00 00 00 00 00 |.STAKTRJ|T.......|
|00000050| 00 00 00 00 00 f6 40 00 | 00 5c 72 a7 ec c9 92 aa |......@.|.\r.....|
|00000060| c4 44 ec 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |.D......|........|
|00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 6b c5 00 00 |........|....k...|
|00000080| 00 00 08 00 53 54 41 4b | ff ff ff ff 00 00 00 00 |....STAK|........|
|00000090| 00 00 00 0a 00 00 f6 40 | 00 00 08 00 00 00 00 00 |.......@|........|
|000000a0| 00 00 00 00 00 00 00 01 | 00 00 0a ab 00 00 00 0a |........|........|
|000000b0| 00 00 0b 21 00 00 13 e8 | 00 00 00 00 00 00 00 00 |...!....|........|
|000000c0| 00 00 0f 32 00 00 00 00 | 00 05 00 00 50 00 00 00 |...2....|....P...|
|000000d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000000e0| 02 10 80 00 02 20 80 00 | 02 20 80 00 02 20 80 00 |..... ..|. ... ..|
|000000f0| 9e 4b fd 8d 00 00 00 00 | 00 28 00 40 01 7e 02 40 |.K......|.(.@.~.@|
|00000100| 00 00 00 00 01 90 02 80 | 00 00 00 00 00 00 00 00 |........|........|
|00000110| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000120| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000130| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000140| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000150| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000160| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000170| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000180| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000190| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000200| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000210| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000220| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000230| 00 00 0d 39 00 00 0c ef | 01 56 02 00 00 00 00 00 |...9....|.V......|
|00000240| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000250| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000260| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000270| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000280| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000290| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000300| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000310| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000320| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000330| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000340| 00 00 00 00 00 00 00 00 | 80 00 00 00 08 00 00 00 |........|........|
|00000350| 88 00 22 00 88 00 22 00 | 88 88 22 22 88 88 22 22 |.."...".|..""..""|
|00000360| 88 aa 22 aa 88 aa 22 aa | cc aa 33 aa cc aa 33 aa |.."...".|..3...3.|
|00000370| ee aa bb aa ee aa bb aa | ee bb bb ee ee bb bb ee |........|........|
|00000380| ff bb ff ee ff bb ff ee | ff bb ff ff ff bb ff ff |........|........|
|00000390| 80 10 02 20 01 08 40 04 | ff ff ff ff ff ff ff ff |... ..@.|........|
|000003a0| 88 22 88 22 88 22 88 22 | 11 22 44 88 11 22 44 88 |."."."."|."D.."D.|
|000003b0| c4 80 0c 68 43 02 30 26 | b1 30 03 1b d8 c0 0c 8d |...hC.0&|.0......|
|000003c0| aa 00 aa 00 aa 00 aa 00 | 88 22 55 22 88 22 55 22 |........|."U"."U"|
|000003d0| 88 55 22 55 88 55 22 55 | 77 dd 77 dd 77 dd 77 dd |.U"U.U"U|w.w.w.w.|
|000003e0| 80 00 00 00 00 00 00 00 | aa 55 aa 55 aa 55 aa 55 |........|.U.U.U.U|
|000003f0| 03 84 48 30 0c 02 01 01 | 82 44 39 44 82 01 01 01 |..H0....|.D9D....|
|00000400| 88 14 22 41 88 41 22 14 | 80 80 41 3e 08 08 14 e3 |.."A.A".|..A>....|
|00000410| 22 04 8c 74 22 17 98 10 | be 80 88 08 eb 08 88 80 |"..t"...|........|
|00000420| 25 c8 32 89 64 24 4c 92 | a2 9c 41 be 2a c9 14 eb |%.2.d$L.|..A.*...|
|00000430| 40 a0 00 00 04 0a 00 00 | 80 40 20 00 02 04 08 00 |@.......|.@ .....|
|00000440| aa 00 80 00 88 00 80 00 | ff 80 80 80 80 80 80 80 |........|........|
|00000450| 08 1c 22 c1 80 01 02 04 | ff 80 80 80 ff 08 08 08 |..".....|........|
|00000460| f8 74 22 47 8f 17 22 71 | bf 00 bf bf b0 b0 b0 b0 |.t"G.."q|........|
|00000470| ff 7f be 5d a2 41 80 00 | fa f5 fa f5 a0 50 a0 50 |...].A..|.....P.P|
|00000480| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000490| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000500| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000510| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000520| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000530| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000540| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000550| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000560| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000570| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000580| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000590| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000600| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000610| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000620| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000630| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000640| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000650| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000660| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000670| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000680| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000690| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000006a0| 00 20 75 73 69 6e 67 20 | 73 74 61 63 6b 20 22 4c |. using |stack "L|
|000006b0| 61 62 48 65 6c 70 65 72 | 22 0d 69 66 20 69 6e 43 |abHelper|".if inC|
|000006c0| 6f 6c 6f 72 20 69 73 20 | 65 6d 70 74 79 20 74 68 |olor is |empty th|
|000006d0| 65 6e 20 70 75 74 20 74 | 72 75 65 20 69 6e 74 6f |en put t|rue into|
|000006e0| 20 69 6e 43 6f 6c 6f 72 | 0d 63 48 43 73 65 74 55 | inColor|.cHCsetU|
|000006f0| 70 20 69 6e 43 6f 6c 6f | 72 2c 20 46 61 6c 73 65 |p inColo|r, False|
|00000700| 2c 20 22 48 43 63 6f 6c | 6f 72 69 7a 65 22 0d 48 |, "HCcol|orize".H|
|00000710| 43 63 6f 6c 6f 72 69 7a | 65 0d 70 61 73 73 20 6f |Ccoloriz|e.pass o|
|00000720| 70 65 6e 53 74 61 63 6b | 0d 65 6e 64 20 6f 70 65 |penStack|.end ope|
|00000730| 6e 53 74 61 63 6b 0d 00 | 2c 20 46 61 6c 73 65 2c |nStack..|, False,|
|00000740| 20 22 48 43 63 6f 6c 6f | 72 69 7a 65 22 0d 48 43 | "HCcolo|rize".HC|
|00000750| 63 6f 6c 6f 72 69 7a 65 | 0d 65 6e 64 20 6f 70 65 |colorize|.end ope|
|00000760| 6e 53 74 61 63 6b 00 74 | 61 72 74 20 75 73 69 6e |nStack.t|art usin|
|00000770| 67 20 73 74 61 63 6b 20 | 22 52 65 63 69 70 65 20 |g stack |"Recipe |
|00000780| 42 6f 6f 6b 22 0d 73 74 | 61 72 74 20 75 73 69 6e |Book".st|art usin|
|00000790| 67 20 73 74 61 63 6b 20 | 22 4c 61 62 50 72 65 70 |g stack |"LabPrep|
|000007a0| 22 0d 73 74 61 72 74 20 | 75 73 69 6e 67 20 73 74 |".start |using st|
|000007b0| 61 63 6b 20 22 4d 65 74 | 68 22 0d 73 74 61 72 74 |ack "Met|h".start|
|000007c0| 20 75 73 69 6e 67 20 73 | 74 61 63 6b 20 22 54 69 | using s|tack "Ti|
|000007d0| 6d 65 20 26 20 44 61 74 | 65 22 0d 73 74 61 72 74 |me & Dat|e".start|
|000007e0| 20 75 73 69 6e 67 20 73 | 74 61 63 6b 20 22 4e 6f | using s|tack "No|
|000007f0| 74 65 73 22 0d 73 74 61 | 72 74 20 75 73 69 6e 67 |tes".sta|rt using|
|00000800| 20 73 74 61 63 6b 20 22 | 50 65 72 69 6f 64 69 63 | stack "|Periodic|
|00000810| 20 54 61 62 6c 65 22 0d | 63 72 65 61 74 65 4d 65 | Table".|createMe|
|00000820| 6e 75 0d 63 48 43 73 65 | 74 55 70 20 54 72 75 65 |nu.cHCse|tUp True|
|00000830| 2c 20 46 61 6c 73 65 2c | 20 22 48 43 63 6f 6c 6f |, False,| "HCcolo|
|00000840| 72 69 7a 65 22 0d 48 43 | 63 6f 6c 6f 72 69 7a 65 |rize".HC|colorize|
|00000850| 0d 65 6e 64 20 6f 70 65 | 6e 53 74 61 63 6b 00 6f |.end ope|nStack.o|
|00000860| 22 20 6f 72 20 22 4f 4b | 22 0d 69 66 20 69 74 3d |" or "OK|".if it=|
|00000870| 22 4f 4b 22 20 74 68 65 | 6e 0d 64 6f 4d 65 6e 75 |"OK" the|n.doMenu|
|00000880| 00 00 02 00 4d 41 53 54 | ff ff ff ff 00 00 00 00 |....MAST|........|
|00000890| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000008a0| 00 00 00 00 00 00 00 00 | 00 00 94 ab 00 02 b0 21 |........|.......!|
|000008b0| 00 07 89 ef 00 07 92 39 | 00 02 a6 74 00 07 95 32 |.......9|...t...2|
|000008c0| 00 07 a0 69 00 03 3d 9c | 00 03 b9 aa 00 00 50 e8 |...i..=.|......P.|
|000008d0| 00 00 54 c5 00 00 00 00 | 00 07 a5 37 00 00 00 00 |..T.....|...7....|
|000008e0| 00 00 00 00 00 06 ee fb | 00 00 00 00 00 00 00 00 |........|........|
|000008f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 03 ec a0 |........|........|
|00000900| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000910| 00 00 00 00 00 04 f0 5a | 00 00 00 00 00 05 30 6d |.......Z|......0m|
|00000920| 00 00 00 00 00 06 22 ae | 00 00 00 00 00 06 78 02 |......".|......x.|
|00000930| 00 00 00 00 00 06 9f 41 | 00 00 00 00 00 00 00 00 |.......A|........|
|00000940| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000950| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000960| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000970| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000980| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000990| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a00| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a20| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a40| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a50| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a80| 00 00 00 80 4c 49 53 54 | 00 00 13 e8 00 00 00 00 |....LIST|........|
|00000a90| 00 00 00 01 00 00 08 00 | 00 00 00 0a 00 84 00 02 |........|........|
|00000aa0| 00 20 00 03 a0 00 02 a2 | 00 00 00 0a 00 00 00 00 |. ......|........|
|00000ab0| 00 00 14 c5 00 0a 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ac0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ad0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ae0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000af0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000b00| 00 00 08 00 50 41 47 45 | 00 00 14 c5 00 00 00 00 |....PAGE|........|
|00000b10| 00 00 13 e8 d9 22 8e 10 | 00 00 0b 21 e0 3b 24 a7 |....."..|...!.;$.|
|00000b20| 8b 7c 7c 00 a1 29 4f 92 | b5 44 9c 06 c3 64 0f 81 |.||..)O.|.D...d..|
|00000b30| d0 39 e3 83 8b d1 90 4d | ea 65 08 36 fc 35 26 ce |.9.....M|.e.6.5&.|
|00000b40| f0 54 f8 94 cf 5c bb ad | de 8f c8 38 ce e3 17 f0 |.T...\..|...8....|
|00000b50| 83 98 d6 20 2c a1 88 5c | 6c 9c cc 62 d6 45 a9 54 |... ,..\|l..b.E.T|
|00000b60| 15 9c 40 17 33 88 7f 71 | 94 69 62 87 48 48 96 50 |..@.3..q|.ib.HH.P|
|00000b70| 47 b3 10 2e 84 8b 42 6d | 0f ea 70 aa ec 85 aa 50 |G.....Bm|..p....P|
|00000b80| a0 4a 84 03 4f c2 d8 50 | 90 00 5d d2 a0 68 30 53 |.J..O..P|..]..h0S|
|00000b90| 67 d0 50 4c 00 f6 30 c8 | 26 74 18 14 00 00 11 9c |g.PL..0.|&t......|
|00000ba0| a0 75 a5 57 97 58 8a 69 | 77 b3 89 3e c5 b6 d0 a6 |.u.W.X.i|w..>....|
|00000bb0| c0 77 df dc fa 9b cd 13 | 99 74 94 6b fa 79 3e 10 |.w......|.t.k.y>.|
|00000bc0| fd 65 ae f3 d4 f7 dd b2 | cf ef fa ad eb 4f fe de |.e......|.....O..|
|00000bd0| 6e 78 77 3f a7 f5 4a f4 | 6f bf ad 6d 4e d3 cd dd |nxw?..J.|o..mN...|
|00000be0| b6 5f fd 47 75 be 7d 3f | 17 e8 43 33 b9 3a b5 17 |._.Gu.}?|..C3.:..|
|00000bf0| da cd 94 5c cc 9f c5 4c | e8 ac a6 3f a7 ee ef f6 |...\...L|...?....|
|00000c00| b1 a5 bf ba 36 72 a6 59 | 7e 7f fc c1 97 1e 5f d2 |....6r.Y|~....._.|
|00000c10| c6 68 70 3f b1 7e f7 d3 | 7e f3 b6 a3 9e 75 3d 0c |.hp?.~..|~....u=.|
|00000c20| 00 00 12 aa a0 08 00 0f | 10 00 a0 00 81 00 19 b1 |........|........|
|00000c30| c1 80 48 04 46 20 02 01 | 40 20 85 02 85 30 00 d9 |..H.F ..|@ ...0..|
|00000c40| 66 49 48 a3 c0 0d 06 00 | 82 00 dc 80 6a 54 f0 0d |fIH.....|....jT..|
|00000c50| ea 54 80 80 c2 16 15 41 | 00 20 40 90 13 0c 29 40 |.T.....A|. @...)@|
|00000c60| 08 54 c9 54 83 06 2a 45 | 13 8a 00 12 1c 80 32 13 |.T.T..*E|......2.|
|00000c70| 11 80 80 14 18 40 c1 01 | 00 04 00 84 84 0a 00 30 |.....@..|.......0|
|00000c80| 84 4a 01 96 8a 88 a8 08 | 12 04 84 0c 55 55 e6 64 |.J......|....UU.d|
|00000c90| 80 00 50 3a 00 80 14 08 | 01 10 12 00 40 c4 06 80 |..P:....|....@...|
|00000ca0| 06 82 00 50 00 00 1f a0 | a0 73 67 7e bf 4f cb d8 |...P....|.sg~.O..|
|00000cb0| 2f bb 9f db ff 7e fd bb | ff be d9 6b de fd ff f6 |/....~..|...k....|
|00000cc0| d9 fb bd fb ee ef ff d5 | fd ff ee fd fe fd dd df |........|........|
|00000cd0| ef e7 fe fd ff df fe fd | ff ff fe ff 6f a5 5f fc |........|....o._.|
|00000ce0| ef 8f ff ff ef ff cf db | b3 fe ef ef ff bf f5 d7 |........|........|
|00000cf0| 37 fb f9 77 df bc be bf | db ed df fd cf ff ef ef |7..w....|........|
|00000d00| dc df ff 3f ff ff ff ef | bf b5 ef ff e2 ff ff 7d |...?....|.......}|
|00000d10| df ff bf f7 ff 2b ff df | c7 7a 7f ff 7d 7f f9 5b |.....+..|.z..}..[|
|00000d20| ea fd b1 bb f3 fe 4f 7c | 00 00 25 5a a0 11 35 2e |......O||..%Z..5.|
|00000d30| 3d 92 f8 49 aa 2b dd b7 | 7d 0e 7d 31 67 6b 9d 00 |=..I.+..|}.}1gk..|
|00000d40| d0 75 d5 73 10 72 97 fc | e0 31 78 b1 f4 0d 06 e3 |.u.s.r..|.1x.....|
|00000d50| 46 50 cc 97 7b 0d 7d eb | ef 8e c7 43 f2 ac bd 57 |FP..{.}.|...C...W|
|00000d60| 66 68 3e 14 ef a4 28 1f | 2f c6 cd 64 5e 6c b7 48 |fh>...(.|/..d^l.H|
|00000d70| c1 5e e0 07 d6 b4 e2 23 | 96 d2 e8 0c 7b 89 a2 4e |.^.....#|....{..N|
|00000d80| cf 3f 85 cf 9c 90 ae 3f | 9f c8 f8 5a bd b1 3a b0 |.?.....?|...Z..:.|
|00000d90| dc 46 a4 45 cc f7 90 d2 | 83 32 fd d8 25 7b f4 f7 |.F.E....|.2..%{..|
|00000da0| 8a bd 59 72 32 de a8 a0 | 16 47 02 58 00 00 27 6d |..Yr2...|.G.X..'m|
|00000db0| a0 7b 5f ff d7 de eb fb | eb e3 bf ff f3 ff ff bf |.{_.....|........|
|00000dc0| df 77 fd bf fa 6f df fb | df f7 ff ff ff ff ff ff |.w...o..|........|
|00000dd0| ff ef 2e fe be 7d ff ff | ff 7f fe fd fa 3e ff 7b |.....}..|.....>.{|
|00000de0| ef 7f df f3 f7 f9 ff d7 | ff fd fd ef 7f ff df ff |........|........|
|00000df0| f3 7d ff ff f7 bf b7 d7 | 7f ae ff 7f ff ff bf df |.}......|........|
|00000e00| fb dd f7 ff bf ff 6e ef | cf bf 7e 3f df ff 7f 8f |......n.|..~?....|
|00000e10| ff b7 ff ff 7f ee ff 5f | 7f fe f9 fd ff 3f ff fa |......._|.....?..|
|00000e20| 85 fb f5 ff fd 7b ff ff | fe ff f6 fb fe 7f 77 7c |.....{..|......w||
|00000e30| 00 00 29 ae a0 53 53 6f | 3c 42 ea 48 2b ab 9f 3f |..)..SSo|<B.H+..?|
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|00006070| 20 6e 75 63 6c 65 69 63 | 20 61 63 69 64 3a 20 20 | nucleic| acid:  |
|00006080| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|00006090| 20 20 20 20 20 b5 67 0d | 45 71 75 69 76 61 6c 65 |     .g.|Equivale|
|000060a0| 6e 74 20 65 6e 64 73 3a | 20 20 20 20 20 20 20 20 |nt ends:|        |
|000060b0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|000060c0| 20 20 20 20 70 6d 6f 6c | 65 0d 43 49 41 50 3a 20 |    pmol|e.CIAP: |
|000060d0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|000060e0| 20 75 20 28 20 20 20 20 | 20 20 20 20 20 75 2f 70 | u (    |     u/p|
|000060f0| 6d 6f 6c 65 20 65 6e 64 | 73 29 0d 0d 0d 0d 31 20 |mole end|s)....1 |
|00006100| b5 67 20 6f 66 20 31 2c | 30 30 30 20 62 70 20 44 |.g of 1,|000 bp D|
|00006110| 4e 41 20 3d 20 31 2e 35 | 32 20 70 6d 6f 6c 65 20 |NA = 1.5|2 pmole |
|00006120| 44 4e 41 0d 28 33 2e 30 | 33 20 70 6d 6f 6c 65 73 |DNA.(3.0|3 pmoles|
|00006130| 20 6f 66 20 65 6e 64 73 | 29 0d 0d 31 20 b5 67 20 | of ends|)..1 .g |
|00006140| 70 42 52 33 32 32 20 44 | 4e 41 20 3d 20 30 2e 33 |pBR322 D|NA = 0.3|
|00006150| 36 20 70 6d 6f 6c 65 20 | 44 4e 41 0d 28 34 2c 33 |6 pmole |DNA.(4,3|
|00006160| 36 33 20 62 70 2c 20 30 | 2e 37 32 20 70 6d 6f 6c |63 bp, 0|.72 pmol|
|00006170| 65 20 65 6e 64 73 29 0d | 0d 00 ff fc 00 06 00 31 |e ends).|.......1|
|00006180| 31 30 30 30 ff f9 00 05 | 00 30 2e 30 31 00 ff fb |1000....|.0.01...|
|00006190| 00 02 00 34 ff f8 00 05 | 00 30 2e 30 31 00 ff fa |...4....|.0.01...|
|000061a0| 00 05 00 31 2e 31 30 00 | 00 1b 05 cb 80 26 00 00 |...1.10.|.....&..|
|000061b0| 00 27 00 10 00 30 00 3b | 00 31 00 e4 00 32 00 e8 |.'...0.;|.1...2..|
|000061c0| 00 31 01 88 00 32 01 8d | 00 31 03 b5 00 32 03 c6 |.1...2..|.1...2..|
|000061d0| 00 31 38 2f 31 35 2f 39 | 34 2c 20 33 3a 34 39 20 |.18/15/9|4, 3:49 |
|000061e0| 41 4d 0d 0d 42 41 50 20 | 6f 72 20 43 49 41 50 20 |AM..BAP |or CIAP |
|000061f0| 44 65 70 68 6f 73 70 68 | 6f 72 79 6c 61 74 69 6f |Dephosph|orylatio|
|00006200| 6e 0d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 0d 42 6f 74 |n.------|----.Bot|
|00006210| 68 20 62 61 63 74 65 72 | 69 61 6c 20 61 6c 6b 61 |h bacter|ial alka|
|00006220| 6c 69 6e 65 20 70 68 6f | 73 70 68 61 74 65 61 73 |line pho|sphateas|
|00006230| 65 20 28 42 41 50 29 20 | 61 6e 64 20 63 61 6c 66 |e (BAP) |and calf|
|00006240| 20 69 6e 74 65 73 74 69 | 6e 61 6c 20 61 6c 6b 61 | intesti|nal alka|
|00006250| 6c 69 6e 65 20 70 68 6f | 73 70 68 61 74 61 73 65 |line pho|sphatase|
|00006260| 20 28 43 49 50 29 20 63 | 61 74 61 6c 79 7a 65 20 | (CIP) c|atalyze |
|00006270| 74 68 65 20 72 65 6d 6f | 76 61 6c 20 6f 66 20 35 |the remo|val of 5|
|00006280| 27 2d 70 68 6f 73 70 68 | 61 74 65 20 72 65 73 69 |'-phosph|ate resi|
|00006290| 64 75 65 73 20 66 72 6f | 6d 20 44 4e 41 2c 20 52 |dues fro|m DNA, R|
|000062a0| 4e 41 2c 20 72 4e 54 50 | 73 20 61 6e 64 20 64 4e |NA, rNTP|s and dN|
|000062b0| 54 50 73 2e 0d 0d 55 53 | 45 53 3a 0d 0d 31 2e 20 |TPs...US|ES:..1. |
|000062c0| 52 65 6d 6f 76 69 6e 67 | 20 35 27 20 70 68 6f 73 |Removing| 5' phos|
|000062d0| 70 68 61 74 65 73 20 66 | 72 6f 6d 20 44 4e 41 20 |phates f|rom DNA |
|000062e0| 6f 72 20 52 4e 41 20 70 | 72 69 6f 72 20 74 6f 20 |or RNA p|rior to |
|000062f0| 6c 61 62 65 6c 69 6e 67 | 20 35 27 20 74 65 72 6d |labeling| 5' term|
|00006300| 69 6e 69 20 77 69 74 68 | 20 dc db 50 2e 0d 0d 32 |ini with| ..P...2|
|00006310| 2e 20 52 65 6d 6f 76 69 | 6e 67 20 35 27 20 70 68 |. Removi|ng 5' ph|
|00006320| 6f 73 70 68 61 74 65 73 | 20 66 72 6f 6d 20 66 72 |osphates| from fr|
|00006330| 61 67 6d 65 6e 74 73 20 | 6f 66 20 44 4e 41 20 74 |agments |of DNA t|
|00006340| 6f 20 70 72 65 76 65 6e | 74 20 73 65 6c 66 2d 6c |o preven|t self-l|
|00006350| 69 67 61 74 69 6f 6e 2e | 0d 0d 4e 6f 74 65 3a 0d |igation.|..Note:.|
|00006360| 0d 42 41 50 20 69 73 20 | 74 68 65 20 6d 6f 72 65 |.BAP is |the more|
|00006370| 20 61 63 74 69 76 65 20 | 6f 66 20 74 68 65 20 74 | active |of the t|
|00006380| 77 6f 20 61 6c 6b 61 6c | 69 6e 65 20 70 68 6f 73 |wo alkal|ine phos|
|00006390| 70 68 61 74 61 73 65 2c | 20 62 75 74 20 69 74 20 |phatase,| but it |
|000063a0| 69 73 20 61 6c 73 6f 20 | 66 61 72 20 6d 6f 72 65 |is also |far more|
|000063b0| 20 72 65 73 69 73 74 61 | 6e 74 20 74 6f 20 68 65 | resista|nt to he|
|000063c0| 61 74 20 61 6e 64 20 64 | 65 74 65 72 67 65 6e 74 |at and d|etergent|
|000063d0| 73 2e 20 49 74 20 69 73 | 20 74 68 65 72 66 6f 72 |s. It is| therfor|
|000063e0| 65 20 64 69 66 66 63 75 | 6c 74 20 74 6f 20 69 6e |e diffcu|lt to in|
|000063f0| 68 69 62 69 74 20 42 41 | 50 20 63 6f 6d 70 6c 65 |hibit BA|P comple|
+--------+-------------------------+-------------------------+--------+--------+
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